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Image Search Results
Journal: medRxiv
Article Title: GDNF levels regulate lumbar motor neuron physiology and determine life expectancy in limb-onset ALS
doi: 10.64898/2025.12.05.25341683
Figure Lengend Snippet: (A) Analysis of RET-positive neurons in the lumbar spinal cord (n=3 animals per group, 6 sections per animal) reveals no difference between the genotypes (B-C) Analyses of NMJs at 35 weeks of age showed significantly more occupied (p=0.0033) NMJs in Gdnf wt/hyper mice and less denervated (p=0.0036) NMJs compared to the Gdnf wt/wt animals (n=5 neurofilament (2H3) and synaptic vesicles (SV2) were stained to visualize the axons and pre-synaptic nerve terminals, α-bungarotoxin (α-BTX) was used to visualize the endplate (stains acetylcholine receptors in skeletal muscle fibers). ( Gdnf wt/wt n=5 and n=6 Gdnf wt/hyper ). (D) Representative images of an NMJ in a 35-week-old Gdnf wt/wt and Gdnf wt/hyper mouse in SOD1 G93A background (scale bar = 10µm). Note the lack of neuronal staining in Gdnf wt/wt in SOD1 G93A background, indicating degeneration of MN axons.
Article Snippet: Muscle sections were blocked with PBS-Triton X-100 (0.5%) and incubated for 24h with anti-SV2 and
Techniques: Staining